The enzyme substrate method is the standard method for E. coli detection included in GB5750-2006. The enzyme substrate method is currently the most advanced method for E. coli detection in water. At present, it is convenient and fast, with low false positives, and is suitable for rapid detection of large numbers of samples. It is gradually being recognized by domestic inspection departments.
Compared with the multi-tube fermentation and membrane filtration method, the detection steps of the enzyme substrate method are greatly reduced, and the requirements for the experimental environment are not high, and the detection time can be reduced to 24 hours. It has a good application prospect in daily water sample monitoring and emergency monitoring. , Can timely detect, prevent, and minimize the occurrence of major public safety accidents.
1. No need to operate in a sterile room.
2. Manual operation time is less than 1 minute.
3. No medium preparation and a large number of glassware sterilization.
4.24 hours to complete qualitative and quantitative analysis without verification test.